Analytical Techniques Online Course

Consortium for Educational Communication (CEC), New Delhi is offering online course for postgraduate students in Analytical Techniques.

About the course:

• The analytical techniques routinely used in Biochemistry take a very important place in Biochemistry, learning them is not just a requirement but, absolutely essential.
• This is because, the understanding of the subject mainly comes from the strong basis of the experiments and techniques on which the theories are built.
• This course deals with various techniques their principles, experimental details etc., including general Laboratory techniques, separation techniques based on hydrodynamic properties, centrifugation (sub-cellular fractionation etc), and Chromatographic, and Electrophortic techniques , HPLC, Purification methods, ELISA etc.
• Application in biomedical research and some clinical diagnostic methods RIA, ELISA, PET etc., Moleular diagnostics ,PCR, etc.,
• Microscopic techniques and clinical biochemistry.

Course Layout:

• Week 1: Aqueous solutions, Acids, Bases, buffers systems and pH meter, Colorimetry and UV-VIS absorption spectroscopy, Introduction to Hydrodynamic Techniques
• Week 2: Centrifugation: Principles & Methodology, Density Gradient centrifugation : Rate Zonal and isopycnic, Differential centrifugation for Sub-cellular Fractionation
• Week 3: Gel filtration: Principle, Methodology & Applications, Planar Chromatography: Principles and Applications
• Week 4: Ion-exchange chromatography, Affinity chromatography
• Week 5: Gas chromatography, HPLC, Protein Estimation Techniques
• Week 6: Assessment-2, Light Microscopy and Confocal Microscopy, Fluorescence Microscopy : Application to live cell imaging
• Week 7: IHC and IF, Flow cytometry: Theory and concept, Flow cytometry: Application in biology and medicine
• Week 8: Electron Microscopy, TEM and SEM,SDS-PAGE
• Week 9: Western Blotting Techniques,2D Electrophoresis and DIGE, Principles of Mass spectrometry
• Week 10: Clinical Proteomics, Methods to quantify and Integrity check of DNA
• Week 11: Methods to quantify and Integrity check of RNA,PCR and Real Time PCR, Blotting techniques for Nucleic acids
• Week 12: Sequencing techniques of nucleic acids, Introduction to Recombinant DNA Technology, Assessment-2
• Week 13: Expression techniques of recombinant proteins using Bacteria and yeast, Expression techniques of recombinant proteins by insects and mammalian cells, Introduction to Genomic Techniques
• Week 14: Techniques for studying Nucleic acid protein interactions, ELISA, RIA :Diagnostic applications
• Week 15: CLIA and its Diagnostic applications, PET and its Diagnostic applications, Single cell biology, Final Examination

Duration: 15 weeks

Enrollment Ends: September 30, 2020

For more details, please visit: https://onlinecourses.swayam2.ac.in/cec20_bt22/preview